The general results matched my results. The same exact procedures and materials were used, but the dependent variables were measured in different concentrations.
The positive The catalase lab essay was the pH 4 test tube, and the negative control was the pH 13 test tube.
Some errors in the lab were both lab errors and human errors. Conclusion and Evaluation6 3. Lastly, it also concludes that catalase prefers neutral pH levels around 7. Conclusion and Evaluation 3. The positive control of the experiment was the test tube with the 1: The results assembled clearly supported all three of my hypotheses.
This lab was completed to analyze and experience how H is decomposed in different concentrations of catalase and H This research will help increase the knowledge of enzyme activity in complex environments, and under what conditions the enzyme catalase will perform best. Systematic Errors The ruler we were using was quite old and dirty, with some of the finer millimeter markings rubbed off or obscured, leading to readings that were not as accurate as they could have been.
In the Substrate concentration experiment, I filled the test tubes with different enzyme to hydrogen peroxide concentrations, which were measured in centimeters. Each test tube was left at the designated temperature for two minutes, and then afterwards were tested every ten seconds for a minute.
Each test tube was left at room temperature and then tested every ten seconds for a minute.
I also assumed that there would be a less drastic change when H was added as oppose to more catalase being added. My first hypothesis is that higher temperatures amplify catalyzation, although too high of a temperature denatures enzymes.
This was done by increasing the number of catalase in the lab with the same amount of H, and then increasing the amount of H with the same amount of catalase to observe results and patterns. Three different experiments were tested in this lab; temperature, substrate concentration and pH.
Suggestions for future work would be to test how the enzyme catalase is able to function in extremophiles and mesophiles, because of their extreme conditions. Graph 1 shows the increased decomposition of H that was produced when the amount of catalase was increased by 2 disks every time.
Both these assumptions were proved correct throughout the lab. Clean and clear rulers should be used to measure the bubbles. The significance of these experiments can be best described as informational data that informs young scientists as well as the general public.
Catalase is a naturally occurring enzyme that breaks down hydrogen peroxide into water and oxygen; it is essential to cellular respiration.Our class began a lab based around enzymes and how they react when different variables are changed, such as temperature, pH, and concentration of the yeast or hydrogen peroxide.
The yeast acted as the enzyme, which produces catalase needed for our desired reaction with the hydrogen peroxide. Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the.
The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity - Catalase Lab introduction. This lab shows how the enzyme decomposes in hydrogen peroxide.
Catalase is a naturally occurring enzyme that breaks down hydrogen peroxide into water and oxygen; it is essential to cellular respiration. I asked if enzyme activity was affected when exposed to different conditions, such as temperature, substrate concentration, and.
- Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis In essence, the main objective was to use chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (H2O2) to water and oxygen by using the enzyme catalase.
Catalase Activity lab report Essay Effect of Temperature on Enzyme Activity Bernard Stepteau Biology Lab /Th am – am Dr Laynes Hypothesis: As the temperature of the enzyme catalase rises the activity of the reaction will decrease.Download